Ratio Spectra First order Derivative Method has been developed for simultaneous estimation of Diclofenac Potassium and Serratiopeptidase. The method involved analytical signals were measured at maxima 266.80 nm and minima 257.0 nm for Diclofenac Potassium and Serratiopeptidase in the first order derivative spectra (∆ λ=8 nm and Scaling factor = 1) of the ratio spectra obtained by using 10 µg/ml SER and 10 µg/ml DP spectra as divisor for Diclofenac Potassium and Serratiopeptidase respectively. Beer’s law obeyed in concentration range of 2-18 µg/ml in methanol for both the drugs. The percent assay of marketed formulation was 99.93±1.0151 and 100.46±0.7790% (2072.84 units/mg) of Diclofenac Potassium and Serratiopeptidase respectively. The proposed methods are specific, sensitive, accurate and precise since they are recommended for routine analysis.
Loading....